Pc10 Manual New |best|: Narishige
According to the Narishige PC-10 User Manual , achieving reproducibility requires careful alignment and environmental awareness:
The PC-10 manual dictates a specific sequence for creating a pipette 1.2.1. Step 1: Single Stage Pulling (For Preliminary Tapering) Set the Mode-selector knob to STEP 1 . Adjust the NO. 1 HEATER level to the desired value. Press the Start switch. Step 2: Two-Stage Pulling (For Finalizing Tips) For a two-stage pull (essential for finer tips): Set the mode to STEP 2 . Set the NO. 1 HEATER level.
: Microinjection needles, deep-tissue delivery, and intracellular recording. Two-Stage (Double) Pull Mode
Disconnect the power. Loosen the terminal screws holding the filament legs. Remove the old coil, insert the new Narishige factory-approved platinum filament, center it visually around a test capillary, and firmly tighten the screws. 7. Troubleshooting Guide Probable Cause Corrective Action Glass does not separate Heater settings are too low. narishige pc10 manual new
Complete User Manual and Operating Guide for the Narishige PC-10 Glass Microelectrode Puller
Visual LEDs illuminate to show which stage is currently active and whether the heater is receiving power. 4. Step-by-Step Operating Instructions
Double-check that the slider is fully engaged in its starting position. Press the button. According to the Narishige PC-10 User Manual ,
: Updated manuals often include enhanced safety precautions and maintenance advice, ensuring users can operate the device safely and efficiently, minimizing wear and tear.
Ensure the glass is perfectly vertical and aligned with the heating filament.
Restricts the travel distance of the first-stage pull, determining how much the glass thins before the final blowout burn. Electronic Control Panel Power Switch: Toggles main system power. 1 HEATER level to the desired value
Insert the filament leads into the terminal blocks, centering the loop perfectly around the vertical glass trajectory path.
Gravity-operated mechanism that provides the force for the pull. 3. Initial Setup and Calibration
Her phone buzzed. A message from New Lab Supplies: the PC-10 was discontinued. Lena’s fingers pressed the controller more tightly. The manual’s language—technician-forward, exacting—felt suddenly more intimate, a testament against obsolescence. It had been a tool for a generation of careful hands, each note in the margins a fingerprint: a tiny smear at “sterilize before use,” a pencil tick by “troubleshooting—air leak,” a dotted line connecting a page number to a memory.
I can provide and step-by-step programming adjustments for your exact workflow. Share public link
Labs that need consistent, simple puller for standard patch pipettes (1–5 MΩ) without complex programming. Excellent for teaching labs or routine recordings.